Why does RBC have no DNA
Red Blood Cell-Ready Gene is the inno-train product line for the analysis of erythrocyte blood group systems using SSP-PCR technology. These diagnostic systems are therefore ideally suited for further analysis of serologically pre-typed samples. The modular kit design gives you the freedom to decide which systems you want to use according to your question. The results are evaluated in the classic way using electrophoresis separation in an agarose gel. As a PCR control, primers for amplifying the HGH (Human Growth Hormone) gene are included in each batch.
The advantages of the Ready Gene System
- High flexibility through modular kit design
- Quick and easy test execution
- Safety in polytransfused patients
- Clarification of questionable RHD / RHCE findings
- Including negative controls
- Colored primer mixes for better visibility
- CE certified
Patients who have a weak AB0 expression, or whose isoagglutinin pattern does not clearly match, can also use RBC-Ready Gene AB0 / AB0 subtype can be post-typed up to very weak antigens. The flexible format of RBC-ready genes enables both tests to be carried out jointly using a parallel approach or exclusively subtyping as required.
Genotyping of rare blood groups
Often, antisera from rare blood groups are either not available or only with difficulty. Patients occasionally develop antibodies that are difficult to identify and which are also directed against antigens from the area of the rare blood group alleles. Means RBC-Ready Gene Rare ID Rare blood group features are detected unequivocally and inexpensively.
In the course of globalization, the demand for blood products with rare blood groups is steadily increasing. Our screening concepts for rare blood group alleles support you in your search for the right canned food. The choice is yours: that RBC-Ready Gene Rare Screen System searches for five different rare blood group alleles in just one reaction. The RBC-Ready Gene 4-Screen The system screens in 4 reactions for 7 different rare blood group alleles and simultaneously checks the Rhesus D-positivity or D-negativity. The samples that tested positive in the screening are then clearly identified with the RBC-Ready Gene Rare ID System.
Genotyping the Kell, Kidd, Duffy, and MNS systems
Various hemolytic diseases such as B. sickle cell anemia or thalassemia, but also complex disease courses with multiple operations after accidents or chronic diseases, require regular transfusions. Transfusions produce blood mixtures in the recipients, which make a post-transfusion determination in particular of the blood groups of the systems Kell, Kidd, Duffy and MNS of the recipient difficult.
If there is a need for further transfusions, the molecular blood group typing provides clear results because the transfused red cell concentrates do not contain any amounts of donor DNA that could influence the test. It also gives you RBC-ready genes Safety in patients who have formed allo- or autoantibodies and / or show a positive DCT in the serological typing.
We offer in addition to the classic RBC-Ready Gene MNS and RBC-Ready Gene KKD Test systems nor that RBC-Ready Gene KELplus Kit for the detection of other Kell alleles, as well as that RBC-Ready Gene JKplusFY for the detection of further Kidd alleles in combination with the Duffy detection.
In cases of clarification of serologically weak D determinations in patients and donors, the inno-train systems RBC-Ready Gene CDE and RBC-Ready Gene D weak can be used individually or in combination. Samples in question are specifically examined for D categories, D partial and D weak and thus clearly characterized.
Molecular biological re-typing of serologically D negative samples with a C or E antigen shows in rare cases an actual D positivity (DEL, D weak or D variant).
The RBC-Ready Gene ZygoFast The test system is recommended as a rapid test for an initial prognosis of the zygosis. You can use four PCRs to analyze whether your sample is "DD", "Dd" or "dd". The complete deletion of the RHD gene leads to the creation of the so-called hybrid box, which is detected by two different reactions to record several polymorphisms. The amplification of two upstream box sequences confirms the presence of the RHD gene.
With the help of RBC-Ready Gene D AddOn System, additional RHD sequences and other D negative alleles are detected that are not based on the deletion of the RHD gene, such as B. DELs, RHDpsi, d (C) es, D-CE (2-9) -D or W16X.
The detection of these alleles provides important additional information for a clear determination of zygotes. All zygotia with known D negative alleles are reliably detected by combining the two kits.
Detailed RHCE detection: RBC-Ready Gene RHCE variants
The RBC-Ready Gene CDE System provides unambiguous results for the RHCE alleles C, c, E, e and C.w. In the case of a clear molecular RHCE determination and a questionable serological result of the C, c, E, e reactions, we recommend the use of our latest test system for clarification RBC-Ready Gene RHCE variants.
It is suitable for analyzing abnormal serological findings such as: B. Unexpected RH antibodies. In addition, the system is suitable for the targeted examination of donors / patients for an altered expression of the RHCE variant alleles.
RBC-Ready Gene vERYfy: The combined solution including VEL + / VEL-
Samples from polytransfused patients or from patients who produce allo- / auto-antibodies usually require a clear molecular investigation. The RBC-Ready Gene vERYfy System offers a combined solution for the parallel examination of the blood group systems Rhesus, MNS, Kell, Kidd, Duffy, Dombrock and Vel.
RBC-Ready Gene Vel-Screen
In 2013, the molecular mechanism of the Vel blood group, which has been described for over 60 years, was deciphered. A homozygous deletion of 17 nucleotides in exon 3 of the SMIM1 gene on chromosome 1p36 causes the rare Vel-negative phenotype.
We offer this for quick and easy Vel screening RBC-Ready Gene Vel-Screen System with only 2 reactions for the Vel + and Vel- genotyping.
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